immunogenicity assessment of brucella mellitensis hsp and tf proteins by immunized rabbit serum.
نویسندگان
چکیده
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منابع مشابه
optimization and efficient purification in production of brucella melitensis recombinant hsp and tf proteins with low endotoxin contents
background the development of an effective subunit vaccine against brucellosis is a research area of intense. but optimization of recombinant proteins production in escherichia coli and content of endotoxins associated with final recombinant proteins are very important. objectives in the present study, expression and purification of brucella melitensis rhsp and rtf were optimized to reduce endo...
متن کاملimmune reactivity of brucella melitensis-vaccinated rabbit serum with recombinant omp31 and dnak proteins.
background and objectives: brucella melitensis infection is still a major health problem for human and cattle in developing countries and the middle east. materials and methods: in this study, in order to screen immunogenic candidate antigens for the development of a brucella subunit vaccine, a cytoplasmic protein (dnak) and an outer membrane protein (omp31) of b. melitensis were cloned, expres...
متن کاملImmune reactivity of Brucella melitensis–vaccinated rabbit serum with recombinant Omp31 and DnaK proteins
BACKGROUND AND OBJECTIVES Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. MATERIALS AND METHODS In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cytoplasmic protein (DnaK) and an outer membrane protein (Omp31) of B. melitensis were cloned, expres...
متن کاملBRUCELLA MELLITENSIS ENDOCARDITIS: DIAGNOSIS AND TREATMENT IN SIX ADULT PATIENTS.
Brucella endocarditis is an uncommon but not rare manifestation of brucellosis in our country. We report six adult patients aged 20 to 45 years. Two of our patients were female, and this is the first time that Brucella endocarditis was reported in adult females. In five cases, aortic valve replacement was performed in addition to combined antibiotic therapy. One of them died due to immedia...
متن کاملAllotypy of Rabbit Serum Proteins
The injection into one rabbit (with Freund's adjuvants) of a specific precipitate made with antibodies from the serum of another rabbit is usually followed by the appearance in the serum of the first rabbit of antibodies which precipitate the serum of certain rabbits but not of others. It was found that the antigen (or one of the antigens) concerned in the reaction of these anti rabbit serum an...
متن کاملبررسی خصوصیات پاسخ شوک حرارتی در بروسلاملی‌تنسیس و واکنش پروتئین شوک حرارتی با سرم افراد بیمار و کنترلC
Background & Aim: Brucella is one of the most important zoonotic diseases and is caused by the members of Brucella genus especially B. melitensis. The bacteria begin to synthesize heat shock proteins(hsp) when facing elevated temperatures. In this investigation, clinical isolates of B. melitensis were subjected to heat shocks and the hsps produced were surveyed by SDS-PAGE electrophoresis. ...
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عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۲، شماره ۲، صفحات ۱۹۲-۱۹۴
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